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2011-07-30 Passing Cells
Passing Cells Supplies: Modified Eagle’s Minimum Essential Medium (MEM; UCSF Cell Culture Facility:#06BL02) · Earle’s Balanced Salt Solution (BSS) · 2 mM L-glutamine ·……
2011-07-30 Cell Maintenance (Feeding Cells)
Cell Maintenance (Feeding Cells) Supplies: Modified Eagle’s Minimum Essential Medium (MEM; UCSF Cell Culture Facility:#06BL02) · Earle’s Balanced Salt Solution (BSS) · ……
2011-07-30 Caco-2 Cell Culture Protocol
Caco-2 Cell Culture Protocol Protocol prepared by: Casey M. Kraning Date: 6/11/07 Media Preparation Supplies: Eagle’s Minimum Essential Medium (MEM; UCSF Cell Culture Facility:#06BL02) ……
2011-07-23 体外细胞的原代培养、传代培养、冻存和复苏
  一、实验原理……
2011-07-01 从脐静脉分离干细胞
从脐静脉分离干细胞 试剂和材料:1.培养基:完全LG-DMEM:含2mmol/L的左旋谷氨酰胺的低糖DMEM,添加10%热灭活胎牛血清,100U/ml青霉素和100μg/ml链霉素;2.A;3.胰蛋白酶,0.05%,含EDTA;4.胶原酶A,0.1%用A配制;5.培养瓶;6.导管;实验方法:1.在正常分娩后6-...……
2011-07-01 Primary Culture of Cortical and Hippocampal neurons
Primary Culture of Cortical and Hiocampal neuro Set-up for the diection:Day 1:Add poly D-lysine/laminin solution to culture dishes/coversli. Swirl the plate to eure that the coating mix covers the entire bottom of the plate. Leave the dishes/cove...……
2011-07-01 不连续Nycodenz梯度溶液中原代细胞溶酶体的分离
不连续Nycodenz梯度溶液中原代细胞溶酶体的分离 试剂和器材:1.匀浆介质:0.25mol/L蔗糖溶液、1mmol/L乙二胺四乙酸、10mmol/L Hepes-NaOH,pH7.4;2.NycoprepTM Universal;3.磷酸缓冲液pH7.4(50mmol/L)4.NycoprepTM Universal稀释剂:6mmol/L乙二胺四乙酸、60mmo...……
2011-07-01 体内DPSCs的成牙和成骨分化
体内DCs的成牙和成骨分化 试剂和材料:1.无镁和钙的平衡盐溶液(A);2.MSC培养基:含2mmol/L 谷氨酰胺的α-MEM,添加15%胎牛血清,0.1mmol/L左旋抗坏血酸磷酸盐,100U/ml青霉素和100μg/ml链霉素;3.胰蛋白酶-EDTA溶液:0.05%胰蛋白酶,0.54mmol/L(0.2%)E...……
2011-07-01 脐带血干细胞的准备
脐带血干细胞的准备试剂和材料:1.运输培养基:Eagle基础培养基(EBM),添加300U/ml青霉素和300μg/ml链霉素,150μg/ml庆大霉素和1μg/ml两性霉素B;2.夹子;3.剪刀;4.抗凝剂:CPDA-1:122mmol/L(26g/L)枸橼酸钠,0.142mol/L(25.5g/L)葡萄糖,15.6 mmo...……
2011-07-01 酶消化法从Wharton胶中分离干细胞
酶消化法从Wharton胶中分离干细胞 试剂和材料:1.培养基:完全LG-DMEM:含2mmol/L的左旋谷氨酰胺的低糖DMEM,添加10%热灭活胎牛血清,100U/ml青霉素和100μg/ml链霉素;2.A;3.胰蛋白酶,2.5%;4.胶原酶A,0.1%用A配制;5.培养瓶;6.圆锥形离心管,50ml;7.剪刀;...……
2011-07-01 Analysis of DNA Fragments of Apoptosis with Gel Electrophoresis
Analysis of DNA Fragments of Apoptosis with Gel Electrophoresi Formation of DNA fragments of oligonucleosomal size (180-200 ) is a hallmark of apoptosis in many cell types.1. Diee 0.5 ml of cell sueion (no le than 5x105, otherwise DNA will not be...……
2011-07-01 DPSCs/SHED的原代培养
DCs/SHED的原代培养 Stem cells from human exfoliated deciduous teeth (SHED)Postnatal human dental pulp stem cells (DCs)试剂和材料:1.无镁和钙的平衡盐溶液(A);2.MSC培养基:含2mmol/L 谷氨酰胺的α-MEM,添加15%胎牛血清,0.1mmol/L左旋抗坏血酸磷酸...……
2011-07-01 Isolation of Mice Muscle Cells
Isolation of Mice Muscle CellThe gastrocnemius, soleus, and plantaris were excised from three C57BL/6-Ly-5.1 6-week-old mice. Tendo, all bone, and fat were carefully discarded, and the muscle tiue was thoroughly minced and then digested at 37&deg...……
2011-07-01 大鼠骨髓原代培养用于生产MSCs
大鼠骨髓原代培养用于生产MSC试剂和材料:1.完全培养基(CCM):α-MEM:α-低限量基础培养基,含谷氨酰胺,无核苷酸或脱氧核苷酸;添加:20%附加L-谷氨酰胺 2mmol/L、经F杂交瘤纯化,非热灭活、青霉素100U/ml、链霉素100μg/ml。含0.2μg/ml两性霉素...……
2011-07-01 收集大鼠骨髓用于生产MSCs
收集大鼠骨髓用于生产MSC 试剂和材料:1.完全培养基(CCM):α-MEM:α-低限量基础培养基,含谷氨酰胺,无核苷酸或脱氧核苷酸;添加:20%附加L-谷氨酰胺 2mmol/L、经F杂交瘤纯化,非热灭活、青霉素100U/ml、链霉素100μg/ml。过滤除菌。储存于4℃,不...……
2011-07-01 Postnatal human dental pulp stem cells (DPSCs)
Postnatal human dental pulp stem cells (DCs) 1.ormal human impacted third molars were collected from adults. 2.Tooth surfaces were cleaned and cut around the cementum-enamel junction by using sterilized dental fiure burs to reveal the pulp chambe...……
2011-07-01 Immunohistochemistry of Postnatal human dental pulp stem cell...
Immunohistochemistry of Postnatal human dental pulp stem cells (DCs)1.rimary DCs and BMSCs were subcultured into 8-chamber slides (2 &time 104 cells/well) (Nunc). 2.The cells were fixed in 4% formalin, and then reacted with saturating levels of p...……
2011-07-01 人骨髓不连续密度梯度离心用于间充质干细胞生产
人骨髓不连续密度梯度离心用于间充质干细胞生产 试剂和材料:1.完全培养基:α-MEM:α-低限量基础培养基,含谷氨酰胺,无核苷酸或脱氧核苷酸;添加:20%附加L-谷氨酰胺 2mmol/L、经F杂交瘤纯化,非热灭活、青霉素100U/ml、链霉素100μg/ml。过滤除菌。...……
2011-07-01 间充质干细胞的传代培养
间充质干细胞的传代培养试剂和材料:1.完全培养基:α-MEM:α-低限量基础培养基,含谷氨酰胺,无核苷酸或脱氧核苷酸;添加:20%附加L-谷氨酰胺 2mmol/L、经F杂交瘤纯化,非热灭活、青霉素100U/ml、链霉素100μg/ml。过滤除菌。储存于4℃,不超过2周;2...……
2011-07-01 MSCs分化为矿化的成骨细胞
MSCs分化为矿化的成骨细胞 试剂和材料:1.完全培养基(CCM):α-MEM:α-低限量基础培养基,含谷氨酰胺,无核苷酸或脱氧核苷酸;添加:20%附加L-谷氨酰胺 2mmol/L、经F杂交瘤纯化,非热灭活、青霉素100U/ml、链霉素100μg/ml。过滤除菌。储存于4℃,不...……

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