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2010-04-19 Northern Blot Procedure
This is a brief overview of how a Northern blot (more formally called an RNA blot) is performed and what type of data you can obtain form one.
2010-04-19 PROTOCOL FOR NORTHERN BLOTTING
PROTOCOL FOR NORTHERN BLOTTING
2010-04-19 Northern Blotting
Northern Blotting
2010-04-19 Northern Blot with Nitrocellulose
Northern Blot with Nitrocellulose
2010-04-19 组织印迹的Northern杂交
1)硝酸纤维素膜或尼龙膜上的组织印迹 2)用35S-标记的RNA探针检测植物印迹中的mRNA
2010-04-19 Northern杂交试验指导手册
一、RNA提取 方法一、改良异硫氰酸胍提取法 方法二、改良Krapp提取法 二、RNA质量检测 方法一、紫外吸收检测 方法 二、变性琼脂糖凝胶电泳检测 三、为制备RNA探针模板的 DNA片断亚克隆 A. 载体选择 B. 目的DNA序列与载体连接 C. 感受态细胞制备(CaCl2>法) D. 质粒DNA转化 E. 转化质粒的快速检测 四、模板DNA的制备 A.质粒DNA的小量提取 B. 质粒DNA的线性化 五、RNA探针制备(根据the DIG system user's guide) A. 地高辛标记的体外转录 方法一、用标准DIG测试条比较鉴定 方法二、用 DIG标记的RNA对照比较检测 六、Northern 印迹杂交
2010-04-19 推荐;Northern blot protocol
2010-04-19 核酸杂交技术(Northern Blot、Southern Blot和探针标记)
(一)Southern Blot 将待检测的DNA分子用/不用限制性内切酶消化后,通过琼脂糖凝胶电泳进行分离,继而将其变性并按其在凝胶中的位置转移到硝酸纤维素薄膜或尼龙膜上,固定后再与同位素或其它标记物标记的DNA或RNA探针进行反应。(二)Northern Blot 在变性条件下将待检的RNA样品进行琼脂糖凝胶电泳,继而按照同Southern Blot相同的原理进行转膜和用探针进行杂交检测。
2010-04-19 Northern杂交操作步骤和DNA探针的制备与标记
Northern 杂交是用来测量真核生物RNA的量和大小估计其丰度的实验方法,可以从大量的RNA样本同时获得这些信息。
2010-04-19 Northern杂交分析操作步骤
2010-04-19 Northern印迹的制备和Northern印迹
Northern印迹的制备和Northern印迹
2010-04-19 RNA点杂交
纯化的RNA的点杂交和狭线杂交 RNA样品准备 RNA样品的印迹和RNA在膜上的固定 固定化RNA的杂交与洗膜
2010-04-19 Northern Blot实验仪器试剂和方法步骤
[仪器、试剂、材料]
2010-04-19 Northern blotting操作步骤
2010-04-16 高分子量RNA杂交
For 1% Agarose Gel; To prepare RNA for loading; Transfer to Nylon membrane (Genescreen Plus/NEN) by capillary action; Transfer to Nylon membrane (Genescreen Plus/NEN) by capillary action
2010-04-16 Northern Blot(northern杂交)
Preparation of Formaldehyde Agarose Gel; Preparation of RNA Samples; Running Conditions; Transfer of RNA to Membrane; Hybridization Conditions; Washing Conditions
2010-04-16 E.coli Total RNA Labeling Protocol for Spotted Microarray
E.coli Total RNA Labeling Protocol for Spotted Microarray
2010-04-16 Northern Blots
We found that both formaldehyde and glyoxal gels work very well for electrophoresis of RNA; we only prefer glyoxal gels because the fumes from the formaldehyde gels are unpleasant. Most protocols suggest using nitrocellulose rather than charged nylon membranes for blotting; the latter are said to give higher background.
2010-04-16 Atlas™cDNA表达距阵(Expression Array)
2010-04-16 总RNA提取与Northern杂交
一、总RNA的提取 二、RNA凝胶和浓度检测 检测RNA的质量:检测18s和28s核糖体RNA,样品含总的RNA-rRNA、tRNA、mRNA。 三、RNA电泳

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